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Recent advances in mouse genetics and the development of very precise assays for cell phenotypes have enabled quantification of the kinetics of growth and turnover of subsets of natural killer (NK) cell populations. Yet, the most important function of NK cells, their ability to lyse tumor cells and infected cells, remains the most difficult to measure. This is due to the fact that a single NK cell can kill tumor cells or virus-infected cells that are targets or resistant to the killing process, respectively. Here, we used the methods of generalized linear models to quantify the contribution of the primary NK cell pool (both mature and immature) and the effector NK cell pool (mature, effector, and immature) to tumor cell destruction by a standard murine cytomegalovirus infection of mice with an intact immune system. We describe a series of models using two YAC T cell receptor gene mutants (LY75.1(+) and LY75.1(-)) as targets to investigate the interaction of host and viral factors controlling the formation of cytotoxic effector molecules from NK cells
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