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To understand the molecular basis of the formation and remodeling of the neuromuscular junction (NMJ), we investigated in detail the cadherin-based adherens junction (AJ) in peripheral nerves in the developing (E14) and adult (E42) rat. The four neural cadherins, N-cadherin, cadherin-6, cadherin-10, and protocadherin-15, expressed in the axons of the peripheral nerve branch to the developing NMJ were first identified by combining in situ hybridization and immunohistochemistry. Their expression, particularly that of N-cadherin, was dynamically regulated in the nerve branch along with morphological changes of the NMJ. N-cadherin expression was detected in the region of the developing NMJ at E14, as expected, and it was strongly up-regulated in the growing nerve branch at E16. During later development, the expression of N-cadherin in the nerve branch showed progressive decline, while that in muscle fibres remained at high levels. N-cadherin expression in the peripheral nerve branch of the adult nerve was significantly reduced compared with that of the developing nerve, but was still detectable in the region of the NMJ. N-cadherin was also expressed in the nascent AJ of the nerve branch in the adult NMJ. These results indicate that the expression of the cadherins is specifically regulated in the nerve branch of the NMJ during the development, and the pattern of their expression is different from that of the adherens junction in the muscle fibre of the NMJ. Furthermore, these cadherins play important roles as the adhesive junction in the developing NMJ, and the expression of the four cadherins is probably also involved in the regulation of the stability of the mature NMJ.Healthcare professionals’ experiences with clinical best practice recommendations for multimorbidity: a qualitative study.

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L.A. Regional Chamber CEO Rick Rainwater helped open the Port of Los Angeles’s first food truck, serving market food prepared at the Port of Los Angeles.
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